Reference Report for SoyBase73101136
| Title: | Detection of five seedborne legume viruses in one sensitive multiplex polymerase chain reaction test |
| Authors: | Bariana, H.S., Shannon, A.L., Chu, P.W.G., Waterhouse, P.M. |
| Source: | Phytopath. 1994, 84(10):1201-1205 |
| Abstract: | A reverse transcription polymerase chain reaction (RT-PCR) assay was developed that can simultaneously test a sample, in 1 tube, for the presence of 5 seedborne legume viruses that are of great concern to legume germplasm banks: alfalfa mosaic alfamovirus, bean yellow mosaic potyvirus, clover yellow vein potyvirus, cucumber mosaic cucumovirus and subterranean clover mottle sobemovirus. RT-PCR assays were also developed for the detection of subterranean clover redleaf luteovirus [?soybean dwarf luteovirus] and subterranean clover stunt virus, thus providing a detection system for all well-characterized viruses known to infect Trifolium subterranean. Primers were designed so that the size of the RT-PCR product was indicative of the virus amplified and, where sequences of more than 1 strain of virus were available, conserved regions were given preference as primer targets. The RT-PCR assay detected all isolates tested for each virus and was up to 5 times more sensitive than ELISA. |
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