Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina-Phaseolina

Reference Report for SoyBase41498400

Title:	Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina-Phaseolina
Authors:	Srivastava, A.K., Arora, D.K.
Source:	Plant Pathol. 1997, 46(5):785-794
Abstract:	Detection and quantification of M. phaseolina, causal agent of charcoal/dry root rot disease in many crop plants, was carried out using ELISA. Polyclonal antisera raised to water soluble extracts of mycelium, the residual water insoluble mycelial materials or ribosomal proteins were evaluated for specificity and cross-reactivity with 16 common soil fungi by Ouchterlony double diffusion (ODD) and double antibody sandwich-ELISA. Soluble and cell wall antisera exhibited strong cross-reactivity with most of the fungal isolates. Ribosomal antibodies were less reactive to common soil fungi except Fusarium oxysporum f.sp. ciceris. Mycelial antigens of M. phaseolina on chickpea (cv. Desi) roots were detectable with DAS-ELISA at a minimum concentration of 10 ng/g at 1:100 root:buffer dilution. Quantitative estimation of M. phaseolina on roots was evaluated by ELISA under different temperatures and moisture conditions and in soil amended with a potential antagonist (Trichoderma harzianum 25-92). A significant reduction in ELISA values was observed in T. harzianum-amended treatments. This method may be useful for detection and rapid screening of M. phaseolina under different environmental conditions

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