Reference Report for SoyBase1118021135
| Title: | Molecular mapping of the fasciation mutation in soybean, Glycine max (Leguminosae) |
| Authors: | Karakaya, H.C., Tang, Y.H., Cregan, P.B., Knap, H.T. |
| Source: | Amer. J. Bot. 2002, 89(4):559-565 |
| Abstract: | The spontaneous fasciation mutation generates novel developmental diversity in cultivated soybean. Glycine max (L.) Merrill. An increased apical dominance in the Mutant inhibits axillary buds, causes a branchless phenotype, and restricts reproduction to shoot apices The fasciation mutation is encoded by a recessive (f) allele at a single locus. The mutation, despite its importance in soybean development, has no locus assignment on previously reported molecular maps of soybean. A Population of 70 F-2 progeny was derived from a cross between 'Clark 63' and the fasciation mutant. More than 700 molecular markers (amplified restriction fragment length polymorphisms [AFLPs], random amplified polymorphic DNAs [RAPDs], restriction fragment length polymorphisms [RFLPs]. and simple sequence repeats [SSRs]) were used in mapping of the fasciation phenotype, Twenty linkage groups (LGs) corresponding to the public soybean molecular map are represented on the Clark 63 x fasciation mutant molecular map that spans 3050 centimorgans (cM). The f locus was mapped on LG D1b+W and linked with two AFLPs and four SSR markers (Satt005, Satt141, Satt600, and Satt703). No linkage was found between the f locus and several cDNA polymorphic loci between the wild type and the mutant. The known map position of the f locus and demonstration of the mutant phenotype from early postembryonic throughout reproductive stages provide an excellent resource for investigations of molecular mechanisms affecting soybean ontogeny |
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