SoyBase Follow us on Twitter @SoyBaseDatabase
Integrating Genetics and Genomics to Advance Soybean Research

Reference Report for RTN20220817.1
Title:Dissecting nematode resistance regions in soybean revealedpleiotropic effect of soybean cyst and reniform nematoderesistance genes
Authors:Usovsky, M, Lakhssassi, N., Patil, G.B., Vuong, T.D., Piya, S., Hewezi, T., Robbins, R.T., Stupar, R.M., Meksem, K., Nguyen, H.T.
Source:Usovsky et al., Plant Genome. 2021;14:e20083
Abstract:Reniform nematode (RN, Rotylenchulus reniformis Linford & Oliveira) has emergedas one of the most important plant parasitic nematodes of soybean [Glycine max (L.)Merr.]. Planting resistant varieties is the most effective strategy for nematode management.The objective of this study was to identify quantitative trait loci (QTL) forRN resistance in an exotic soybean line, PI 438489B, using two linkage maps constructedfrom the Universal Soybean Linkage Panel (USLP 1.0) and next-generationwhole-genome resequencing (WGRS) technology. Two QTL controlling RN resistancewere identified—the soybean cyst nematode (SCN, Heterodera glycines) resistancegene GmSNAP18 at the rhg1 locus and its paralog GmSNAP11. Strong associationbetween resistant phenotype and haplotypes of the GmSNAP11 and GmSNAP18was observed. The results indicated that GmSNAP11 possibly could have epistaticeffect on GmSNAP18, or vice versa, with the presence of a significant correlation inRN resistance of rhg1-a GmSNAP18 vs. rhg1-b GmSNAP18. Most importantly, ourpreliminary data suggested that GmSNAP18 and GmSNAP11 proteins physicallyinteract in planta, suggesting that they belong to the same pathway for resistance.Unlike GmSNAP18, no indication of GmSNAP11 copy number variation was found.Moreover, gene-based single nucleotide polymorphism (SNP) markers were developedfor rapid detection of RN or SCN resistance at these loci. Our analysis substantiatessynergic interaction between GmSNAP11 and GmSNAP18 genes and confirmstheir roles in RN as well as SCN resistance. These results could contribute to a betterunderstanding of evolution and subfunctionalization of genes conferring resistanceto multiple nematode species and provide a framework for further investigations.






Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA
 
USDA Logo
Iowa State University Logo