Reference Report for RTN20171229.4
Title: | Novel FAD3 Mutant Allele Combinations Produce Soybeans Containing 1% Linolenic Acid in the Seed Oil |
Authors: | Bilyeu, K., Gillman, J.D., LeRoy, A.R. |
Source: | Crop Sci. 2011, 51:259-264 |
Abstract: | Soybean [Glycine max (L.) Merr.] oil typically contains about 7% of linolenic acid, an oxida- tively unstable fatty acid that is undesirable for many food applications. For cooking oil, reduc- tion of the linolenic acid content by partial hydro- genation produces trans fatty acids, which are now known to increase the risk of coronary heart disease. Genetic reduction of linolenic acid was achieved decades ago, and recently the molecu- lar genetic basis for both 3% and 1% linolenic acid soybean germplasm was revealed to be combinations of mutations in three independent members of the soybean omega-3 fatty acid desaturase (FAD3) genes that corresponded to the fan1, fan2, and fan3 loci. When the mutant GmFAD3A and GmFAD3C genes are found in combination in the breeding line CX1512-44, the linolenic acid content is reduced to approxi- mately 3% of the of the seed oil. When the mutant GmFAD3A and GmFAD3B genes are in combination in the breeding line RG-10, a similar fatty acid profile is produced with approximately 3% linolenic acid. The objective of this work was to determine the ability of different combinations of mutant FAD3 alleles from CX1512-44 and an RG-10?derived soybean line to produce less than 3% linolenic acid in the seed oil. The results indicated that novel combinations of mutations in the three FAD3 genes are capable of produc- ing soybean seeds containing only 1% linolenic acid in the seed oil. |