Effect of various additives on the preservation of southern bean mosaic virus.

Reference Report for IND86050288

Title:	Effect of various additives on the preservation of southern bean mosaic virus.
Authors:	Fukumoto, F., Tochihara, H.
Source:	Ann. Phytopath. Soc. Japan 1985, 51(4):413-420
Abstract:	Freezing for a duration of one hour followed by thawing prevented changes in the infectivity of a purified preparation of southern bean mosaic virus (SBMV) suspended in 10mM potassium phosphate buffer (P.B.), pH 7.0. Infectivity of purified SBMV decreased gradually during the storage under frozen conditions at -20.degree. C. However, the infectivity [on soybean] was maintained at a high level for 52 months by the addition of peptone or glycerol, etc. At -70.degree. C, the infectivity was maintained for a long period without any additives. The virions of purified SBMV freeze-dried without additives became swollen by rehydration with P.B., and their infectivity decreased to 16%. But, the changes in the conformation of SBMV virions due to freeze-drying could be significantly prevented by the addition of lysine. The protective effect of additives during preservation of freeze-dried preparations was assessed at 65.degree. C. The infectivity of freeze-dried preparations in P.B. disappeared in one day but it could be detected 7 days after preservation, when the virus was freeze-dried with lysine.

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