Reference Report for IND82074760
Title: | Some properties of an isolate of the soybean stunt strain of cucumber mosaic virus. |
Authors: | Hanada, K., Tochihara, H. |
Source: | Phytopath. 1982, 72(7):761-764 |
Abstract: | An isolate of soybean stunt virus (SSV-A) obtained from mottled soybean seeds was charactrerized and compared with isolates of cucumber mosaic virus (CMV). SSV-A had a similar host range to that of several CMV strains, although some differences were detected. In agar gel diffusion tests, spur formation was observed between purified SSV-A and 2 serologically different CMV. Polyacrylamide gel electrophoresis (PAGE) showed that SSV-A contained 5 major RNA species that had almost the same MW of CMV RNA. A mixture of the 3 largest RNA of SSV-A had the highest infectivity. SSV-A had 1 kind of protein with a MW of .apprx. 24,000, which comigrated with CMV-protein in PAGE. Pseudorecombinants were constructed between SSV-A and CMV by exchange of RNA 3. Evidently, SSV-A is a soybean strain of CMV with a unique serotype. |