Reference Report for IND22057423
| Title: | A novel promoter from soybean that is active in a complex developmental pattern with and without its proximal 650 base pairs |
| Authors: | Stromvik, M.V., Sundararaman, V.P., Vodkin, L.O. |
| Source: | Plant Mol. Biol. 1999, 41(2):217-231 |
| Abstract: | The isolation is reported of a novel soyabean (Glycine max) gene, Msg (GenBank/EMBL/DDBJ accession number AJ239127), which is highly expressed in developing soyabean pods. The gene shows significant homology to a family of fruit- and flower-specific genes, designated the major latex protein (MLP) homologues, so far reported in only a few species and whose functions are unknown. The MLPs are more distantly related to a group of pathogenesis-related proteins (IPR or PR-10) whose functions are also unknown. This is the first report of an MLP homologue in a plant for which there is already an IPR protein reported. The Msg promoter was analysed using 14 promoter fragments ranging from 0.65 to 2.26 kb, fused to the uidA (GUS) gene. High transient expression was obtained with all the constructs upon particle bombardment in soyabean and green bean (Phaseolus vulgaris) pods. Stable Arabidopsis thaliana transformants were obtained with the Agrobacterium tumefaciens vacuum infiltration method. The promoter is fully active in Arabidopsis only in plants transformed with the 2.26 kb fragment promoter, expressing GUS in nectaries, nodes, short style and in guard cells of the siliqua, pedicel and stem but not in mature leaves. The proximal 650 bp TATA-containing region cannot function on its own in Arabidopsis and can be deleted without a change in expression pattern in both Arabidopsis and soyabean. Thus, tissue-specific regions of the complex Msg promoter reside in the distal 5′ regions upstream of a dispensable TATA box in contrast to many examples of tissue-specific elements that reside much closer to the TATA box. |
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