Reference Report for IND20589487
| Title: | Map order and linkage distances of molecular markers close to the supernodulation (nts-1) locus of soybean. |
| Authors: | Kolchinsky, A., Landau-Ellis, D., Gresshoff, P.M. |
| Source: | Mol. Gen. Genet. 254(1):29-36 |
| Abstract: | The molecular characteristics of markers in the chromosome region surrounding the supernodulation gene (nts-1) of soybean (Glycine max L. Merr.) were investigated in 187 F2 plants from a cross of G. Max cv. Bragg (nts) and G.soja PI468.397 (wild-type nodulation). RFLP marker pUTG-132a, linked tightly (0.7 +/- 0.5 cM) to nts-1, was converted to a PCR marker. The polymorphism resides within a 1.72 kb PstI fragment and consists of an 832 bp insertion in G. max relative to the wild progenitor G. soja. The insertion is flanked by a 35 bp direct duplication that was found only once in G. soja. Data suggest that the pUTG-132a sequence exists only once in the genome, which is compatible with the recessive nature of nts1. Accordingly, pUTG132a is a valuable marker for map-based cloning. Another RFLP marker, pA-381, was mapped 4.8 cM distal to nts-1. Marker order, established by Maximum Likelihood Analysis, placed nts-1 between pUTG-132a and pA-381. To generate additional molecular markers, a segregating F2 population was analysed using bulked segregant analysis (BSA) and single oligonucleotide primer-based PCR (DNA amplification fingerprinting; DAF). PCR marker pcr5-4L was mapped to soybean linkage group H and sequenced. The data revealed (I) recombination events and marker order in the nts-1 region: (ii) The molecular nature and cause of polymorphisms in linked molecular markers; (iii) a low density of polymorphisms around nts-1, and (iv) diploidy of the distal region of linkage group H of soybean. |
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