Root cortical cell spherical bodies associated with an induced resistance reaction in monoxenic cultures of Meloidogyne incognita.

Reference Report for IND20513756

Title:	Root cortical cell spherical bodies associated with an induced resistance reaction in monoxenic cultures of Meloidogyne incognita.
Authors:	Orion, D., Wergin, W.P., Chitwood, D.J.
Source:	J. Nematol. 1995, 27(3):320-327
Abstract:	The root-knot nematode Meloidogyne incognita was monoxenically cultured on excised roots of soybean cv. Pickett and tomato cv. Rutgers in agar media containing either 0 to 1,600 microgram/ml ammonium nitrate or 0 to 100 microgram/ml urea. Observations with scanning and transmission electron microscopy indicated that an elevated concentration of ammonium nitrate or urea inhibited giant cell formation and suppressed nematode development in the infected soybean roots. In the tomato roots, concentrations of ammonium nitrate above 400 microgram/ml or urea above 25 microgram/ml inhibited giant cell formation and nematode development. Coincident with the nitrogen concentrations that suppressed giant cell formation, was the appearance of electron-dense spherical bodies in the cortical parenchyma cells of both the soybean and tomato roots. These bodies, which were 1-4 micrometer in diameter, appeared to form in the cytoplasm and migrate to the cell vacuole.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

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