Reference Report for IND20397452
| Title: | Production of cercosporin and colonization of soybean seed coats by Cercospora kikuchii |
| Authors: | Velicheti, R.K., Sinclair, J.B. |
| Source: | Plant Dis. 1994, 78(4):342-346 |
| Abstract: | Isolates of C. kikuchii produced cercosporin in culture and soyabean seed coats. C. kikuchii isolates recovered from purple-stained soyabean seeds were divided into 3 groups based on colony coloration on potato-dextrose agar (PDA). Ckcp isolates produced purple coloration, Ckcy isolates a yellow background due to lipids, and Ckcc isolates red cercosporin. Of 72 isolates from 72 seeds studied, 75% were Ckcp, 13% Ckcy and 12% Ckcc. One isolate representing each group was studied further. The Ckcp isolate produced significantly higher amounts of cercosporin on PDA than either the Ckcy or Ckcc isolates. Production of cercosporin by the 3 isolates in seed coats was in the order of Ckcc > Ckcp > Ckcy. All isolates inoculated on soyabean seeds in culture caused purple stain on the seed coats. The extent of the purple staining coincided with the extent of colonization of C. kikuchii. A positive relationship (r² = 0.96) was found between the extent of purple seed coat stain and the amount of cercosporin per 100 µg of seed coat tissue in situ. Extracts from C. kikuchii grown on PDA contained cercosporin, isocercosporin and lipids. However, lipids were not detected from soyabean seed coats inoculated with C. kikuchii or naturally infected purple-stained seed coats, suggesting that the pathogen produced lipids only in culture and that lipids were not involved in seed colonization. This is the first report on the estimation of cercosporin from Cercospora in plant tissues with reference to pathogenesis. It is suggested that cercosporin has a role in facilitating soyabean seed coat tissue colonization by C. kikuchii as there was a greater occurrence of isolates producing high amounts of cercosporin and there was a correlation between cercosporin production and the extent of purple stain of C. kikuchii colonization |
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