Reference Report for AP20210709.1
| Title: | Overexpression of soybean GmPLD-gamma enhances seed oil content and modulates fatty acid composition in transgenic Arabidopsis |
| Authors: | Bai, Y., Jing, G., Zhou, J., Li, S., Bi, R., Zhao, J., Jia, Q., Zhang, Q., Zhang, W. |
| Source: | Bai et al. 2020 Plant Science, 290: 110298 |
| Abstract: | Phospholipase D (PLD) hydrolyzes the phosphodiester bond of glycerophospholipids to yield phosphatidic acid (PA) and a free headgroup. PLDs are important for plant growth, development, and responses to external stresses. However, their roles in triacylglycerol (TAG) synthesis are still unclear. Here, we report that a soybean (Glycine max) PLD (GmPLD) is involved in glycerolipid turnover and seed oil production. GmPLD was targeted to mitochondria and exhibited PLD activity that was activated by oleate and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]. Overexpression of GmPLD (abbreviated GmPLD-OE) in Arabidopsis thaliana resulted in enhanced seed weight, elevated levels of TAGs with 18-, 20-, and 22-carbon fatty acids (FAs), and altered oil-body morphology. Furthermore, the levels of membrane lipids in vegetative tissues decreased significantly, whereas no overt changes were found in mature seeds except for a decrease in the digalactosyldiacylglycerol (DGDG) level in the GmPLD-OE lines. Additionally, the expression of genes involved in glycerolipid metabolism was significantly upregulated in developing siliques in GmPLD-OE lines. Together, our data indicate a regulatory role for GmPLD in TAG synthesis and fatty-acid remodeling, highlighting the importance of mitochondria-directed glycerophospholipid homeostasis in seed oil accumulation. |
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