Genome-wide survey and comprehensive expression profiling of Aux/IAA gene family in chickpea and soybean.

Reference Report for AP20210701.1

Title:	Genome-wide survey and comprehensive expression profiling of Aux/IAA gene family in chickpea and soybean.
Authors:	Singh, V.K., Jain, M.
Source:	Singh et al. 2015 Front. Plant Sci., 6: 918
Abstract:	Auxin plays a central role in many aspects of plant growth and development.Auxin/Indole-3-Acetic Acid(Aux/IAA) genes cooperate with several other components in the perception and signaling of plant hormone auxin. An investigation of chickpea and soybean genomes revealed 22 and 63 putativeAux/IAAgenes, respectively. These genes were classified into six subfamilies on the basis of phylogenetic analysis. Among 63 soybeanAux/IAAgenes, 57 (90.5%) were found to be duplicated via whole genome duplication (WGD)/segmental events. Transposed duplication played a significant role in tandem arrangements between the members of different subfamilies. Analysis of Ka/Ks ratio of duplicatedAux/IAAgenes revealed purifying selection pressure with restricted functional divergence. Promoter sequence analysis revealed severalcis-regulatory elements related to auxin, abscisic acid, desiccation, salt, seed, and endosperm, indicating their role in development and stress responses. Expression analysis of chickpea and soybeanAux/IAAgenes in various tissues and stages of development demonstrated tissue/stage specific differential expression. In soybean, at least 16 paralog pairs, duplicated via WGD/segmental events, showed almost indistinguishable expression pattern, but eight pairs exhibited significantly diverse expression patterns. Under abiotic stress conditions, such as desiccation, salinity and/or cold, manyAux/IAAgenes of chickpea and soybean revealed differential expression. qRT-PCR analysis confirmed the differential expression patterns of selectedAux/IAAgenes in chickpea. The analyses presented here provide insights on putative roles of chickpea and soybeanAux/IAAgenes and will facilitate elucidation of their precise functions during development and abiotic stress responses.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

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