Genome-wide analysis of the TCP transcription factor genes in five legume genomes and their response to salt and drought stress

Reference Report for AP20210624.4

Title:	Genome-wide analysis of the TCP transcription factor genes in five legume genomes and their response to salt and drought stress
Authors:	Ling, L., Zhang, W., An, Y., Du, B., Wang, D., Guo, C.
Source:	Ling et al. 2020
Abstract:	The teosinte branched1, cycloidea, and proliferating cell factor family (TCP) proteins, plant-specific transcription factors, are involved in the regulation of plant development; however, theTCPgene family of legumes has been based primarily on a single crop. Here, a total of 55, 22, 26, 21, and 25 genes containing theVQmotif were identified from the genomes ofGlycine max,Cicer arietinum,Phaseolus vulgaris,Medicago truncatula, andLotus japonicus, respectively. Based on the phylogenetic analysis, we divided theseTCPgenes into three distinct subfamilies:PCF,CYC/TB1, andCIN. The conserved domain analysis indicated that theTCPgene family members contain the bHLH and R domains. TheTCPgenes from the same evolutionary branches of legumes shared similar motifs and structures. The promoter analysis revealed thatcis-elements were related to stress responses, phytohormone responses, and physical and reproductive growth regulation. In addition, theTCPgenes presented different expression patterns in the five legumes. Most of them showed specific expression patterns during development. The results of qRT-PCR indicated that theTCPgenes played regulatory roles in both salt and drought stresses. The present study provides novel and detailed information regarding the legumeTCPgene family, which aids in functional characterisation of theTCPgenes in other plants.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

U.S. Public Domain