Reference Report for AP20210624.2
| Title: | Identification of active VQ motif-containing genes and the expression patterns under low nitrogen treatment in soybean. |
| Authors: | Wang, X., Zhang, H., Sun, G., Jin, Y., Qiu, L. |
| Source: | Wang et al. 2015 Gene, 543(2):237-243 |
| Abstract: | Plant VQ motif-containing protein family plays crucial roles in plant growth, seed development, and defense responses in Arabidopsis. However, its function in soybean is still not well defined. We aim to identify theVQgene family, and explore the genetic variation of activeGmVQgenes in soybean and their expression patterns under low nitrogen stresses. A total of 74 VQ motif-containing genes were identified in soybean genome, and were clustered into five distinct subfamilies (GmVQIV) with each gene having two or three copies exceptGmVQ55(GmVQIV) with single copy. Fourteen genes with relatively high expression level, at least in one tissue, were defined as activeGmVQgenes. Most of these activeGmVQgenes specifically expressed in soybean pod shell (7/74), root (9/74) and/or nodule (10/74) respectively. Single nucleotide polymorphism (SNP) analysis in cultivated and wild soybeans revealed there were selected site(s) inGmVQ6,GmVQ7,GmVQ10,GmVQ26andGmVQ61, which means that these genes have undergone artificial selection during soybean domestication. After low nitrogen treatment, enhanced expression ofVQgenes was noticed in specific tissues, such asGmVQ53,GmVQ26,GmVQ58,GmVQ61,GmVQ70andGmVQ6in shoot, andGmVQ53,GmVQ58,GmVQ48in root. On the contrary, suppressed expression ofGmVQ57,GmVQ21andGmVQ1genes was noticed in root after the treatment. Duplicated copy of the activeGmVQgenes showed similar expression pattern, suggesting that these genes might be complete copies. The results suggested that soybean VQ-motif containing genes may act as positive or negative regulators in soybean growth, development and nitrogen metabolism. Taken together, our results provided useful information for functional characterization of soybeanGmVQgenes to unravel their biological roles. |
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