| Title: | A combinatorial action of GmMYB176 and GmbZIP5 controls isoflavonoid biosynthesis in soybean (Glycine max) |
| Authors: | Vadivel, A.K.A., McDowell, T., Renaud, J.B., Dhaubhadel, S., Sangi, S., Araújo, P.M., Coelho, F.S., Gazara, R.K., Almeida-Silva, F., Venacio, T. M., Gravitol, C. |
| Source: | Commun Biol. 4:356 (2021) |
| Abstract: | GmMYB176 is an R1 MYB transcription factor that regulates multiple genes in the iso-flavonoid biosynthetic pathway, thereby affecting their levels in soybean roots. WhileGmMYB176 is important for isoflavonoid synthesis, it is not sufficient for the function andrequires additional cofactor(s). The aim of this study was to identify the GmMYB176 inter-actome for the regulation of isoflavonoid biosynthesis in soybean. Here, we demonstrate thata bZIP transcription factor GmbZIP5 co-immunoprecipitates with GmMYB176 and showsproteinprotein interactionin planta. RNAi silencing ofGmbZIP5reduced the isoflavonoid levelin soybean hairy roots. Furthermore, co-overexpression ofGmMYB176andGmbZIP5enhanced the level of multiple isoflavonoid phytoallexins including glyceollin, isowighteoneand a unique O-methylhydroxy isoflavone in soybean hairy roots. Thesefindings could beutilized to develop biotechnological strategies to manipulate the metabolite levels either toenhance plant defense mechanisms or for human health benefits in soybean or other eco-nomically important crops. |
| Title: | Genome-wide analysis of the COBRA-like gene family supports gene expansion through whole-genome duplication in soybean (Glycine max). |
| Authors: | Vadivel, A.K.A., McDowell, T., Renaud, J.B., Dhaubhadel, S., Sangi, S., Araújo, P.M., Coelho, F.S., Gazara, R.K., Almeida-Silva, F., Venacio, T. M., Gravitol, C. |
| Source: | Sangi et al. Plants 2021, 10:167 |
| Abstract: | The COBRA-like (COBL) gene family has been associated with the regulation of cell wall expansion and cellulose deposition. COBL mutants result in reduced levels and disorganized deposition of cellulose causing defects in the cell wall and inhibiting plant development. In this study, we report the identification of 24 COBL genes (GmCOBL) in the soybean genome. Phylogenetic analysis revealed that the COBL proteins are divided into two groups, which differ by about 170 amino acids in the N-terminal region. The GmCOBL genes were heterogeneously distributed in 14 of the 20 soybean chromosomes. This study showed that segmental duplication has contributed significantly to the expansion of the COBL family in soybean during all Glycine-specific whole-genome duplication events. The expression profile revealed that the expression of the paralogous genes is highly variable between organs and tissues of the plant. Only 20% of the paralogous gene pairs showed similar expression patterns. The high expression levels of some GmCOBLs suggest they are likely essential for regulating cell expansion during the whole soybean life cycle. Our comprehensive overview of the COBL gene family in soybean provides useful information for further understanding the evolution and diversification of COBL genes in soybean. |
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