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Integrating Genetics and Genomics to Advance Soybean Research
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| Title: | Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing |
| Authors: | Qi, X., Li, M., Xie, M., Liu, X., Ni, M., Shao, G., Song, C., Yim, A.K., Tao, Y., Wong, F., Isobe, S., Wong, C., Wong, K., Xu, C., Li, C., Wang, Y., Guan, R., Sun, F., Fan, G., Xiao, Z., Zhou, F., Phang, T., Liu, X., Tong, S., Chan, T., Yiu, S., Tabata, S., Wang, J., Xu, X., Lam, M. |
| Source: | Nat. Commun. 5:4340 (2014) |
| Abstract: | Using a whole-genome-sequencing approach to explore germplasm resources can serve as an important strategy for crop improvement, especially in investigating wild accessions that may contain useful genetic resources that have been lost during the domestication process. Here we sequence and assemble a draft genome of wild soybean and construct a recombinant inbred population for genotyping-by-sequencing and phenotypic analyses to identify multiple QTLs relevant to traits of interest in agriculture. We use a combination ofde novosequencing data from this work and our previous germplasm re-sequencing data to identify a novel ion transporter gene,GmCHX1, and relate its sequence alterations to salt tolerance. Rapid gain-of-function tests show the protective effects ofGmCHX1towards salt stress. This combination of whole-genomede novosequencing, high-density-marker QTL mapping by re-sequencing and functional analyses can serve as an effective strategy to unveil novel genomic information in wild soybean to facilitate crop improvement. |
| Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA | ||
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