Fast Neutron Mutants

Compiled here are over 20,000 independent soybean fast neutron mutant lines derived from M92-220 developed for use in soybean functional genomics. Within the catalog are mutants with observed phenotypic variation in seed composition, maturity, morphology, pigmentation, roots and nodulation. This population was first used for a forward screen of seed protein and oil mutants. In the meantime, it was revitalized as a resource for phenome analysis and future forward and reverse genetics screens. Analysis of genomic DNA from a subset of mutants for deletions and additions by comparative genome hybridization are also displayed on this site. This database is open to the community to search and browse for soybean mutants of interest. Locations of detected DNA deletions and additions may be viewed by checking the "Mutants" track on the SoyBase Wm82 genome sequence browser. The availability of seed from individual mutant lines is indicated. Bulk seed from the fast neutron mutagenesis population is available on request.

Citations

Bolon et al. 2011
Phenotypic and genomic analyses of a fast neutron mutant population resource in soybean.
Publication available from Plant Physiology

Bolon et al. 2014
Genome Resilience and Prevalence of Segmental Duplications Following Fast Neutron Irradiation of Soybean.
Publication available from Genetics

Funding Sources

The National Science Foundation, the U.S. Department of Agriculture-Agricultural Research Service, the Minnesota Soybean Research and Promotion Council, and the United Soybean Board.