Soybean cDNA library Gm-c1047


Clones from this library are available from
    Biogenetic Services
    801 32nd Ave.
    Brookings, SD 57006 USA
    phone: 800 423 4163
    email: info@biogeneticservices.com

To insure that credit is given to the laboratory and scientists donating this valuable resource to the scientific community, the information provided with the library must be maintained and included with any transfer of biological materials or data derived from the library.

Source of Library (Donor):
Dr. Lila O. Vodkin       Phone:   217 244 6147       e-mail:   l-vodkin@uiuc.edu
University of Illinois at Champaign-Urbana              
Department of Crop Science        
384 Edward R. Madigan Laboratory        
1201 W. Gregory Drive        
Urbana, IL 61801        

Short Description of Library:
The cDNA library was constructed from mRNA isolated from immature leaves (unfurled trifoliate) of greenhouse grown plants that were 2 week old. The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a Not I restrictions site. Sal I linkers adapters were ligated to the blunt-ended cDNA fragments followed by Not I digestion. The cDNA fragments were directionally cloned into the Not I-Sal I restriction site of the pSPORT 1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells.

Transformation efficiency: 105
Percent white colonies: 2%
Average insert size of white colonies: 830 bp

Genotype/Cultivar:
Williams

Organ/Tissue:
immature leaves (unfurled trifoated), 2 week old, greenhouse grown

Vector Information:
The cDNA library was prepared using the pSPORT 1 vector (Life Technologies) to generate a plasmid library. The vector provides ampicillin resistance and allows blue-white color selection for recombinant plasmids. The vector also contains unique Sal I and Not I sites needed to clone cDNA directionally. The cloned cDNA fragment can be amplified by the polymerase chain reaction using one of the following vector primer pair combinations: T7 and SP6 or M13 universal and M13 reverse.


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