Soybean cDNA library Gm-r1030


Clones from this library are available from
    Biogenetic Services
    801 32nd Ave.
    Brookings, SD 57006 USA
    phone: 800 423 4163
    email: info@biogeneticservices.com

To insure that credit is given to the laboratory and scientists donating this valuable resource to the scientific community, the information provided with the library must be maintained and included with any transfer of biological materials or data derived from the library.

Library Gm-r1030 is a filter hybridization-driven, reracked set of clones from the original library Gm-c1007. Clones were chosen to increase the representation of low expression cDNAs from Gm-c1007. The Gm-r1030 sequences are from the 5' end of the clones.

Source of Library (Donor):
Dr. Lila O. Vodkin       Phone:   217 244 6147       e-mail:   l-vodkin@uiuc.edu
University of Illinois at Champaign-Urbana              
Department of Crop Science        
384 Edward R. Madigan Laboratory        
1201 W. Gregory Drive        
Urbana, IL 61801        

Short Description of Library:
The cDNA library was constructed from mRNA isolated from immature cotyledons of greenhouse grown plants (individual seed fresh weight of 100-300 mg). The library was prepared using the Life Technologies pSuperscript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly (dT) sequence with a Not I restriction site. Sal I linker adapters were ligated to the blunt-ended cDNA fragements followed by Not I digestion. The cDNA fragements were directionally cloned into the NotI-Sal I restriciton sites of the pSPORT 1 vector. The ligated cDNA fragments were transformed into E. coli Electro Max DH10B host cells.

Genotype/Cultivar:
Williams

Organ/Tissue:
Immature cotyledons, greenhouse grown

Vector Information:
The cDNA library was prepared using the pSPORT 1 vector (Life Technologies) to generate a plasmid library. The vector provides ampicillin resistance and allows blue-white color selection for recombinant plasmids. The vector also contains unique Sal I and Not I sites needed to clone cDNA directionally. The cloned cDNA fragemnt can be amplified by the polymerase chain reaction using one of the following vector primer pair combinations: M13 universal and M13 reverse or T7 and SP6.

Miscellaneous Information:
100 ul of the enclosed dilution of the library will give 2000 colonies. Please plate on X-gal/IPTG plates. Of 20 clones picked to date and characterized by PCR, all had inserts of average size of 1300bp.

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100 ul of the enclosed dilution of the library will give 2000 colonies. Please plate on X-gal/IPTG plates. Of 20 clones picked to date and characterized by PCR, all had inserts of average size of 1300bp.